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KMID : 1094720100150050882
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 5 p.882 ~ p.889
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Optimization of nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20
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Geetha K.
Gunasekaran P.
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Abstract
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We aimed to optimize a nutrient medium containing agricultural waste for xylanase production by Bacillus pumilus B20. Xylanase production with lignocellulosic material was optimized in two steps using DeMeo’s fractional factorial design. A 3.4-fold increase in xylanase production (313.3 U/mL) was achieved using the optimized culture medium consisting of (g/L): K2HPO4, 2; MgSO4·7H2O, 0.3; CaCl2·2H2O, 0.01; NaCl, 2; peptone, 5 yeast extract, 4; and wheat bran, 50. B. pumilus B20 produced a high level of xylanase, which may have potential industrial application.
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KEYWORD
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Bacillus, fractional factorial design, media optimization, wheat bran, xylanase
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